affinity chromatographies การใช้

• However, HPLC techniques exist that do utilize affinity chromatography properties.
• Affinity chromatography often isolates and purifies in a single step.
• It also does not reduce metals used in immobilized metal affinity chromatography.
• Pierce Award by International organization of Affinity chromatography and Biorecognition.
• Many membrane proteins are glycoproteins and can be purified by lectin affinity chromatography.
• Following affinity chromatography, the purified protein is then treated with TEV protease.
• Some of the methods are similar to affinity chromatography by use of immobilized ligands.
• ICAT-labeled peptides were isolated using affinity chromatography . 163 proteins were identified.
• The fragments can be purified by gel filtration, ion exchange, or affinity chromatography.
• Some variants of affinity immunoelectrophoresis are similar to affinity chromatography by use of immobilized ligands.
• Neurocalcin was purified from the bovine brain by using calcium-dependent drug affinity chromatography.
• An additional approach for further purification uses affinity chromatography . transformed ) into the researched bacteria.
• This is why they are used in gas chromatography, thin layer chromatography and affinity chromatography.
• A much quicker, single-step method of separation is protein A / G affinity chromatography.
• The phospholipase activity can be separated by affinity chromatography, using a phospholipid analog ( PC-Sepharose ).
• Promising results were obtained in 2003 in a study that merged the newer developments in affinity chromatography with microfluidic devices.
• It is also the source of concanavalin A, a lectin used in biotechnology applications, such as lectin affinity chromatography.
• For affinity chromatography, beaded agarose is the most commonly used matrix resin for the attachment of the ligands that bind protein.
• Affinity chromatography often utilizes a biomolecule's affinity for a metal ( Zn, Cu, Fe, etc . ).
• One of the applications of imidazole is in the purification of His-tagged proteins in immobilised metal affinity chromatography ( IMAC ).